Publications

The following publications showcase the research taking place in the Ploegh Lab. This list updates automatically as new articles become available on PubMed.


2016 Publications

Phenotypic lentivirus screens to identify functional single domain antibodies.
Schmidt FI, Hanke L, Morin B, Brewer R, Brusic V, Whelan SP, Ploegh HL. Nat Microbiol, 1(8). 2016 Jun 20. PMID: 27573105

Posttranscriptional Regulation of Glycoprotein Quality Control in the Endoplasmic Reticulum Is Controlled by the E2 Ub-Conjugating Enzyme UBC6e.
Hagiwara M, Ling J, Koenig PA, Ploegh HL. Mol. Cell, 63(5). 2016 Sep 1. PMID: 27570074

Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83.
von Rohrscheidt J, Petrozziello E, Nedjic J, Federle C, Krzyzak L, Ploegh HL, Ishido S, Steinkasserer A, Klein L. J. Exp. Med., 213(9). 2016 Aug 22. PMID: 27503071

Structures of TorsinA and its disease-mutant complexed with an activator reveal the molecular basis for primary dystonia.
Demircioglu FE, Sosa BA, Ingram J, Ploegh HL, Schwartz TU. Elife, 5. 2016 Aug 04. PMID: 27490483

Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose.
Chahal JS, Khan OF, Cooper CL, McPartlan JS, Tsosie JK, Tilley LD, Sidik SM, Lourido S, Langer R, Bavari S, Ploegh HL, Anderson DG. Proc. Natl. Acad. Sci. U.S.A., 113(29). 2016 Jul 19. PMID: 27382155

Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute-phase Toxoplasma gondii burden.
Sanecka A, Yoshida N, Dougan SK, Jackson J, Shastri N, Ploegh H, Blanchard N, Frickel EM. Immunology, 149(3). 2016 Nov. PMID: 27377596

Recent advances in sortase-catalyzed ligation methodology.
Antos JM, Truttmann MC, Ploegh HL. Curr. Opin. Struct. Biol., 38. 2016 Jun. PMID: 27318815

Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture.
Dura B, Servos MM, Barry RM, Ploegh HL, Dougan SK, Voldman J. Proc. Natl. Acad. Sci. U.S.A., 113(26). 2016 Jun 28. PMID: 27303033

The Caenorhabditis elegans Protein FIC-1 Is an AMPylase That Covalently Modifies Heat-Shock 70 Family Proteins, Translation Elongation Factors and Histones.
Truttmann MC, Cruz VE, Guo X, Engert C, Schwartz TU, Ploegh HL. PLoS Genet., 12(5). 2016 May. PMID: 27138431

Durable antitumor responses to CD47 blockade require adaptive immune stimulation.
Sockolosky JT, Dougan M, Ingram JR, Ho CC, Kauke MJ, Almo SC, Ploegh HL, Garcia KC. Proc. Natl. Acad. Sci. U.S.A., 113(19). 2016 May 10. PMID: 27091975

A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly.
Schmidt FI, Lu A, Chen JW, Ruan J, Tang C, Wu H, Ploegh HL. J. Exp. Med., 213(5). 2016 May 2. PMID: 27069117

Molecular basis of caspase-1 polymerization and its inhibition by a new capping mechanism.
Lu A, Li Y, Schmidt FI, Yin Q, Chen S, Fu TM, Tong AB, Ploegh HL, Mao Y, Wu H. Nat. Struct. Mol. Biol., 23(5). 2016 May. PMID: 27043298

Corrigendum: CEACAM1 regulates TIM-3-mediated tolerance and exhaustion.
Huang YH, Zhu C, Kondo Y, Anderson AC, Gandhi A, Russell A, Dougan SK, Petersen BS, Melum E, Pertel T, Clayton KL, Raab M, Chen Q, Beauchemin N, Yazaki PJ, Pyzik M, Ostrowski MA, Glickman JN, Rudd CE, Ploegh HL, Franke A, Petsko GA, Kuchroo VK, Blumberg RS. Nature, 536(7616). 2016 Mar 16. PMID: 26982724

Crystal structure of a substrate-engaged SecY protein-translocation channel.
Li L, Park E, Ling J, Ingram J, Ploegh H, Rapoport TA. Nature, 531(7594). 2016 Mar 17. PMID: 26950603

Corrigendum: Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.
Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL. Nat. Biotechnol., 34(2). 2016 Feb. PMID: 26849523

Structurally Defined αMHC-II Nanobody-Drug Conjugates: A Therapeutic and Imaging System for B-Cell Lymphoma.
Fang T, Duarte JN, Ling J, Li Z, Guzman JS, Ploegh HL. Angew. Chem. Int. Ed. Engl., 55(7). 2016 Feb 12. PMID: 26840214

Usp12 stabilizes the T-cell receptor complex at the cell surface during signaling.
Jahan AS, Lestra M, Swee LK, Fan Y, Lamers MM, Tafesse FG, Theile CS, Spooner E, Bruzzone R, Ploegh HL, Sanyal S. Proc. Natl. Acad. Sci. U.S.A., 113(6). 2016 Feb 9. PMID: 26811477

Enzyme-Mediated Modification of Single-Domain Antibodies for Imaging Modalities with Different Characteristics.
Rashidian M, Wang L, Edens JG, Jacobsen JT, Hossain I, Wang Q, Victora GD, Vasdev N, Ploegh H, Liang SH. Angew. Chem. Int. Ed. Engl., 55(2). 2016 Jan 11. PMID: 26630549



2015 Publications

The use of (18)F-2-fluorodeoxyglucose (FDG) to label antibody fragments for immuno-PET of pancreatic cancer.
Rashidian M, Keliher E, Dougan M, Juras PK, Cavallari M, Wojtkiewicz GR, Jacobsen J, Edens JG, Tas JM, Victora G, Weissleder R, Ploegh H. ACS Cent Sci, 1(3). 2015 Jun 24. PMID: 26955657

Enzyme-Mediated Modification of Single-Domain Antibodies for Imaging Modalities with Different Characteristics.
Rashidian M, Wang L, Edens JG, Jacobsen JT, Hossain I, Wang Q, Victora GD, Vasdev N, Ploegh H, Liang SH. Angew. Chem. Int. Ed. Engl., 55(2). 2016 Jan 11. PMID: 26630549

Graphene Oxide Nanosheets Modified with Single-Domain Antibodies for Rapid and Efficient Capture of Cells.
Chen GY, Li Z, Theile CS, Bardhan NM, Kumar PV, Duarte JN, Maruyama T, Rashidfarrokh A, Belcher AM, Ploegh HL. Chemistry, 21(48). 2015 Nov 23. PMID: 26472062

Disruption of Sphingolipid Biosynthesis Blocks Phagocytosis of Candida albicans.
Tafesse FG, Rashidfarrokhi A, Schmidt FI, Freinkman E, Dougan S, Dougan M, Esteban A, Maruyama T, Strijbis K, Ploegh HL. PLoS Pathog., 11(10). 2015 Oct. PMID: 26431038

Allosteric activation of apicomplexan calcium-dependent protein kinases.
Ingram JR, Knockenhauer KE, Markus BM, Mandelbaum J, Ramek A, Shan Y, Shaw DE, Schwartz TU, Ploegh HL, Lourido S. Proc. Natl. Acad. Sci. U.S.A., 112(36). 2015 Sep 8. PMID: 26305940

Editorial overview: Special section: Immunological engineering.
Irvine D, Ploegh H. Curr. Opin. Immunol., 35. 2015 Aug. PMID: 26275317

Fluorophore-Conjugated Holliday Junctions for Generating Super-Bright Antibodies and Antibody Fragments.
Li Z, Theile CS, Chen GY, Bilate AM, Duarte JN, Avalos AM, Fang T, Barberena R, Sato S, Ploegh HL. Angew. Chem. Int. Ed. Engl., 54(40). 2015 Sep 28. PMID: 26252716

A new TLR2 agonist promotes cross-presentation by mouse and human antigen presenting cells.
Santone M, Aprea S, Wu TY, Cooke MP, Mbow ML, Valiante NM, Rush JS, Dougan S, Avalos A, Ploegh H, De Gregorio E, Buonsanti C, D'Oro U. Hum Vaccin Immunother, 11(8). 2015. PMID: 26024409

Noninvasive imaging of immune responses.
Rashidian M, Keliher EJ, Bilate AM, Duarte JN, Wojtkiewicz GR, Jacobsen JT, Cragnolini J, Swee LK, Victora GD, Weissleder R, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 112(19). 2015 May 12. PMID: 25902531

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining.
Maruyama T, Dougan SK, Truttmann MC, Bilate AM, Ingram JR, Ploegh HL. Nat. Biotechnol., 33(5). 2015 May. PMID: 25798939

Structural biology. Structural basis for chemokine recognition and activation of a viral G protein-coupled receptor.
Burg JS, Ingram JR, Venkatakrishnan AJ, Jude KM, Dukkipati A, Feinberg EN, Angelini A, Waghray D, Dror RO, Ploegh HL, Garcia KC. Science, 347(6226). 2015 Mar 6. PMID: 25745166

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems.
Witte MD, Wu T, Guimaraes CP, Theile CS, Blom AE, Ingram JR, Li Z, Kundrat L, Goldberg SD, Ploegh HL. Nat Protoc, 10(3). 2015 Mar. PMID: 25719269

Toxoplasma gondii superinfection and virulence during secondary infection correlate with the exact ROP5/ROP18 allelic combination.
Jensen KD, Camejo A, Melo MB, Cordeiro C, Julien L, Grotenbreg GM, Frickel EM, Ploegh HL, Young L, Saeij JP. MBio, 6(2). 2015 Feb 24. PMID: 25714710

HypE-specific nanobodies as tools to modulate HypE-mediated target AMPylation.
Truttmann MC, Wu Q, Stiegeler S, Duarte JN, Ingram J, Ploegh HL. J. Biol. Chem., 290(14). 2015 Apr 3. PMID: 25678711

Evasion of innate cytosolic DNA sensing by a gammaherpesvirus facilitates establishment of latent infection.
Sun C, Schattgen SA, Pisitkun P, Jorgensen JP, Hilterbrand AT, Wang LJ, West JA, Hansen K, Horan KA, Jakobsen MR, O'Hare P, Adler H, Sun R, Ploegh HL, Damania B, Upton JW, Fitzgerald KA, Paludan SR. J. Immunol., 194(4). 2015 Feb 15. PMID: 25595793

Profiling lymphocyte interactions at the single-cell level by microfluidic cell pairing.
Dura B, Dougan SK, Barisa M, Hoehl MM, Lo CT, Ploegh HL, Voldman J. Nat Commun, 6. 2015 Jan 13. PMID: 25585172

Intracellular expression of camelid single-domain antibodies specific for influenza virus nucleoprotein uncovers distinct features of its nuclear localization.
Ashour J, Schmidt FI, Hanke L, Cragnolini J, Cavallari M, Altenburg A, Brewer R, Ingram J, Shoemaker C, Ploegh HL. J. Virol., 89(5). 2015 Mar. PMID: 25540369

tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae.
Damon JR, Pincus D, Ploegh HL. Mol. Biol. Cell, 26(2). 2015 Jan 15. PMID: 25392298

CEACAM1 regulates TIM-3-mediated tolerance and exhaustion.
Huang YH, Zhu C, Kondo Y, Anderson AC, Gandhi A, Russell A, Dougan SK, Petersen BS, Melum E, Pertel T, Clayton KL, Raab M, Chen Q, Beauchemin N, Yazaki PJ, Pyzik M, Ostrowski MA, Glickman JN, Rudd CE, Ploegh HL, Franke A, Petsko GA, Kuchroo VK, Blumberg RS. Nature, 517(7534). 2015 Jan 15. PMID: 25363763

One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism.
Swee LK, Lourido S, Bell GW, Ingram JR, Ploegh HL. ACS Chem. Biol., 10(2). 2015 Feb 20. PMID: 25360987



2014 Publications

Intracellular expression of camelid single-domain antibodies specific for influenza virus nucleoprotein uncovers distinct features of its nuclear localization.
Ashour J, Schmidt FI, Hanke L, Cragnolini J, Cavallari M, Altenburg A, Brewer R, Ingram J, Shoemaker C, Ploegh HL. J. Virol., 89(5). 2015 Mar. PMID: 25540369

Herman Eisen (1918-2014).
Steiner L, Ploegh H. Nature, 516(7529). 2014 Dec 4. PMID: 25471875

tRNA thiolation links translation to stress responses in Saccharomyces cerevisiae.
Damon JR, Pincus D, Ploegh HL. Mol. Biol. Cell, 26(2). 2015 Jan 15. PMID: 25392298

Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity.
Wagner K, Kwakkenbos MJ, Claassen YB, Maijoor K, Böhne M, van der Sluijs KF, Witte MD, van Zoelen DJ, Cornelissen LA, Beaumont T, Bakker AQ, Ploegh HL, Spits H. Proc. Natl. Acad. Sci. U.S.A., 111(47). 2014 Nov 25. PMID: 25385586

Intestinal colonization by Candida albicans alters inflammatory responses in Bruton's tyrosine kinase-deficient mice.
Strijbis K, Yilmaz OH, Dougan SK, Esteban A, Gröne A, Kumamoto CA, Ploegh HL. PLoS ONE, 9(11). 2014. PMID: 25379804

CEACAM1 regulates TIM-3-mediated tolerance and exhaustion.
Huang YH, Zhu C, Kondo Y, Anderson AC, Gandhi A, Russell A, Dougan SK, Petersen BS, Melum E, Pertel T, Clayton KL, Raab M, Chen Q, Beauchemin N, Yazaki PJ, Pyzik M, Ostrowski MA, Glickman JN, Rudd CE, Ploegh HL, Franke A, Petsko GA, Kuchroo VK, Blumberg RS. Nature, 517(7534). 2015 Jan 15. PMID: 25363763

One-step enzymatic modification of the cell surface redirects cellular cytotoxicity and parasite tropism.
Swee LK, Lourido S, Bell GW, Ingram JR, Ploegh HL. ACS Chem. Biol., 10(2). 2015 Feb 20. PMID: 25360987

The E2 ubiquitin-conjugating enzyme UBE2J1 is required for spermiogenesis in mice.
Koenig PA, Nicholls PK, Schmidt FI, Hagiwara M, Maruyama T, Frydman GH, Watson N, Page DC, Ploegh HL. J. Biol. Chem., 289(50). 2014 Dec 12. PMID: 25320092

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS. Cell, 159(3). 2014 Oct 23. PMID: 25307932

Site-specific chemoenzymatic labeling of aerolysin enables the identification of new aerolysin receptors.
Wuethrich I, Peeters JG, Blom AE, Theile CS, Li Z, Spooner E, Ploegh HL, Guimaraes CP. PLoS ONE, 9(10). 2014. PMID: 25275512

Negative self-regulation of TLR9 signaling by its N-terminal proteolytic cleavage product.
Lee S, Kang D, Ra EA, Lee TA, Ploegh HL, Park B. J. Immunol., 193(7). 2014 Oct 1. PMID: 25187653

Protein quality control in the endoplasmic reticulum.
Koenig PA, Ploegh HL. F1000Prime Rep, 6. 2014. PMID: 25184039

How lamina-associated polypeptide 1 (LAP1) activates Torsin.
Sosa BA, Demircioglu FE, Chen JZ, Ingram J, Ploegh HL, Schwartz TU. Elife, 3. 2014. PMID: 25149450

GPR107, a G-protein-coupled receptor essential for intoxication by Pseudomonas aeruginosa exotoxin A, localizes to the Golgi and is cleaved by furin.
Tafesse FG, Guimaraes CP, Maruyama T, Carette JE, Lory S, Brummelkamp TR, Ploegh HL. J. Biol. Chem., 289(35). 2014 Aug 29. PMID: 25031321

Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes.
Shi J, Kundrat L, Pishesha N, Bilate A, Theile C, Maruyama T, Dougan SK, Ploegh HL, Lodish HF. Proc. Natl. Acad. Sci. U.S.A., 111(28). 2014 Jul 15. PMID: 24982154

Secretion of circular proteins using sortase.
Strijbis K, Ploegh HL. Methods Mol. Biol., 1174. 2014. PMID: 24947375

The interest in various immunological subdisciplines waxes and wanes with every new discovery. Preface.
Ploegh HL. Adv. Immunol., 123. 2014. PMID: 24840950

B-cell receptor signaling in lymphoid malignancies and autoimmunity.
Avalos AM, Meyer-Wentrup F, Ploegh HL. Adv. Immunol., 123. 2014. PMID: 24840946

Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly.
Abdi K, Singh NJ, Spooner E, Kessler BM, Radaev S, Lantz L, Xiao TS, Matzinger P, Sun PD, Ploegh HL. J. Immunol., 192(12). 2014 Jun 15. PMID: 24821971

The protein synthesis inhibitor blasticidin s enters mammalian cells via leucine-rich repeat-containing protein 8D.
Lee CC, Freinkman E, Sabatini DM, Ploegh HL. J. Biol. Chem., 289(24). 2014 Jun 13. PMID: 24782309

A mouse monoclonal antibody against Alexa Fluor 647.
Wuethrich I, Guillen E, Ploegh HL. Monoclon Antib Immunodiagn Immunother, 33(2). 2014 Apr. PMID: 24746152

Epithelial-to-mesenchymal transition activates PERK-eIF2α and sensitizes cells to endoplasmic reticulum stress.
Feng YX, Sokol ES, Del Vecchio CA, Sanduja S, Claessen JH, Proia TA, Jin DX, Reinhardt F, Ploegh HL, Wang Q, Gupta PB. Cancer Discov, 4(6). 2014 Jun. PMID: 24705811

Early BCR Events and Antigen Capture, Processing, and Loading on MHC Class II on B Cells.
Avalos AM, Ploegh HL. Front Immunol, 5. 2014. PMID: 24653721

The chaperone BAG6 captures dislocated glycoproteins in the cytosol.
Claessen JH, Sanyal S, Ploegh HL. PLoS ONE, 9(3). 2014. PMID: 24594942

Monovalent engagement of the BCR activates ovalbumin-specific transnuclear B cells.
Avalos AM, Bilate AM, Witte MD, Tai AK, He J, Frushicheva MP, Thill PD, Meyer-Wentrup F, Theile CS, Chakraborty AK, Zhuang X, Ploegh HL. J. Exp. Med., 211(2). 2014 Feb 10. PMID: 24493799

In vivo discovery of immunotherapy targets in the tumour microenvironment.
Zhou P, Shaffer DR, Alvarez Arias DA, Nakazaki Y, Pos W, Torres AJ, Cremasco V, Dougan SK, Cowley GS, Elpek K, Brogdon J, Lamb J, Turley SJ, Ploegh HL, Root DE, Love JC, Dranoff G, Hacohen N, Cantor H, Wucherpfennig KW. Nature, 506(7486). 2014 Feb 6. PMID: 24476824

A catalytic independent function of the deubiquitinating enzyme USP14 regulates hippocampal synaptic short-term plasticity and vesicle number.
Walters BJ, Hallengren JJ, Theile CS, Ploegh HL, Wilson SM, Dobrunz LE. J. Physiol. (Lond.), 592(4). 2014 Feb 15. PMID: 24218545



2013 Publications

Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo.
Nair N, Buti L, Faenzi E, Buricchi F, Nuti S, Sammicheli C, Tavarini S, Popp MW, Ploegh H, Berti F, Pizza M, Castellino F, Finco O, Rappuoli R, Del Giudice G, Galli G, Bardelli M. Immun Inflamm Dis, 1(1). 2013 Oct. PMID: 25400913

Logic of the immune system.
Ploegh HL. Cancer Immunol Res, 1(1). 2013 Jul. PMID: 24777244

Transnuclear TRP1-specific CD8 T cells with high or low affinity TCRs show equivalent antitumor activity.
Dougan SK, Dougan M, Kim J, Turner JA, Ogata S, Cho HI, Jaenisch R, Celis E, Ploegh HL. Cancer Immunol Res, 1(2). 2013 Aug. PMID: 24459675

Impaired autophagy in the lipid-storage disorder Niemann-Pick type C1 disease.
Sarkar S, Carroll B, Buganim Y, Maetzel D, Ng AH, Cassady JP, Cohen MA, Chakraborty S, Wang H, Spooner E, Ploegh H, Gsponer J, Korolchuk VI, Jaenisch R. Cell Rep, 5(5). 2013 Dec 12. PMID: 24290752

Type I interferon imposes a TSG101/ISG15 checkpoint at the Golgi for glycoprotein trafficking during influenza virus infection.
Sanyal S, Ashour J, Maruyama T, Altenburg AF, Cragnolini JJ, Bilate A, Avalos AM, Kundrat L, García-Sastre A, Ploegh HL. Cell Host Microbe, 14(5). 2013 Nov 13. PMID: 24237697

A catalytic independent function of the deubiquitinating enzyme USP14 regulates hippocampal synaptic short-term plasticity and vesicle number.
Walters BJ, Hallengren JJ, Theile CS, Ploegh HL, Wilson SM, Dobrunz LE. J. Physiol. (Lond.), 592(4). 2014 Feb 15. PMID: 24218545

A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.
Reiling JH, Olive AJ, Sanyal S, Carette JE, Brummelkamp TR, Ploegh HL, Starnbach MN, Sabatini DM. Nat. Cell Biol., 15(12). 2013 Dec. PMID: 24185178

Antigen-specific B-cell receptor sensitizes B cells to infection by influenza virus.
Dougan SK, Ashour J, Karssemeijer RA, Popp MW, Avalos AM, Barisa M, Altenburg AF, Ingram JR, Cragnolini JJ, Guo C, Alt FW, Jaenisch R, Ploegh HL. Nature, 503(7476). 2013 Nov 21. PMID: 24141948

Production of unnaturally linked chimeric proteins using a combination of sortase-catalyzed transpeptidation and click chemistry.
Witte MD, Theile CS, Wu T, Guimaraes CP, Blom AE, Ploegh HL. Nat Protoc, 8(9). 2013 Sep. PMID: 23989675

Site-specific N-terminal labeling of proteins using sortase-mediated reactions.
Theile CS, Witte MD, Blom AE, Kundrat L, Ploegh HL, Guimaraes CP. Nat Protoc, 8(9). 2013 Sep. PMID: 23989674

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions.
Guimaraes CP, Witte MD, Theile CS, Bozkurt G, Kundrat L, Blom AE, Ploegh HL. Nat Protoc, 8(9). 2013 Sep. PMID: 23989673

Stochastic cytokine expression induces mixed T helper cell States.
Fang M, Xie H, Dougan SK, Ploegh H, van Oudenaarden A. PLoS Biol., 11(7). 2013 Jul. PMID: 23935453

PIKfyve, a class III PI kinase, is the target of the small molecular IL-12/IL-23 inhibitor apilimod and a player in Toll-like receptor signaling.
Cai X, Xu Y, Cheung AK, Tomlinson RC, Alcázar-Román A, Murphy L, Billich A, Zhang B, Feng Y, Klumpp M, Rondeau JM, Fazal AN, Wilson CJ, Myer V, Joberty G, Bouwmeester T, Labow MA, Finan PM, Porter JA, Ploegh HL, Baird D, De Camilli P, Tallarico JA, Huang Q. Chem. Biol., 20(7). 2013 Jul 25. PMID: 23890009

A reporter screen in a human haploid cell line identifies CYLD as a constitutive inhibitor of NF-κB.
Lee CC, Carette JE, Brummelkamp TR, Ploegh HL. PLoS ONE, 8(7). 2013. PMID: 23861985

Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to Dectin1-dependent phagocytosis of Candida albicans in macrophages.
Strijbis K, Tafesse FG, Fairn GD, Witte MD, Dougan SK, Watson N, Spooner E, Esteban A, Vyas VK, Fink GR, Grinstein S, Ploegh HL. PLoS Pathog., 9(6). 2013. PMID: 23825946

Amino acid copolymers that alleviate experimental autoimmune encephalomyelitis in vivo interact with heparan sulfates and glycoprotein 96 in APCs.
Koenig PA, Spooner E, Kawamoto N, Strominger JL, Ploegh HL. J. Immunol., 191(1). 2013 Jul 1. PMID: 23740953

Orthogonal labeling of M13 minor capsid proteins with DNA to self-assemble end-to-end multiphage structures.
Hess GT, Guimaraes CP, Spooner E, Ploegh HL, Belcher AM. ACS Synth Biol, 2(9). 2013 Sep 20. PMID: 23713956

Acidic amino acid residues in the juxtamembrane region of the nucleotide-sensing TLRs are important for UNC93B1 binding and signaling.
Kim J, Huh J, Hwang M, Kwon EH, Jung DJ, Brinkmann MM, Jang MH, Ploegh HL, Kim YM. J. Immunol., 190(10). 2013 May 15. PMID: 23585677

Intact sphingomyelin biosynthetic pathway is essential for intracellular transport of influenza virus glycoproteins.
Tafesse FG, Sanyal S, Ashour J, Guimaraes CP, Hermansson M, Somerharju P, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 110(16). 2013 Apr 16. PMID: 23576732

Intercellular trafficking of the nuclear oncoprotein DEK.
Saha AK, Kappes F, Mundade A, Deutzmann A, Rosmarin DM, Legendre M, Chatain N, Al-Obaidi Z, Adams BS, Ploegh HL, Ferrando-May E, Mor-Vaknin N, Markovitz DM. Proc. Natl. Acad. Sci. U.S.A., 110(17). 2013 Apr 23. PMID: 23569252

Sialylneolacto-N-tetraose c (LSTc)-bearing liposomal decoys capture influenza A virus.
Hendricks GL, Weirich KL, Viswanathan K, Li J, Shriver ZH, Ashour J, Ploegh HL, Kurt-Jones EA, Fygenson DK, Finberg RW, Comolli JC, Wang JP. J. Biol. Chem., 288(12). 2013 Mar 22. PMID: 23362274

Catch-and-release probes applied to semi-intact cells reveal ubiquitin-specific protease expression in Chlamydia trachomatis infection.
Claessen JH, Witte MD, Yoder NC, Zhu AY, Spooner E, Ploegh HL. Chembiochem, 14(3). 2013 Feb 11. PMID: 23335262

Sortase-mediated modification of αDEC205 affords optimization of antigen presentation and immunization against a set of viral epitopes.
Swee LK, Guimaraes CP, Sehrawat S, Spooner E, Barrasa MI, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 110(4). 2013 Jan 22. PMID: 23297227

Monovalent and multivalent ligation of the B cell receptor exhibit differential dependence upon Syk and Src family kinases.
Mukherjee S, Zhu J, Zikherman J, Parameswaran R, Kadlecek TA, Wang Q, Au-Yeung B, Ploegh H, Kuriyan J, Das J, Weiss A. Sci Signal, 6(256). 2013 Jan 1. PMID: 23281368

A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation.
Sehrawat S, Koenig PA, Kirak O, Schlieker C, Fankhauser M, Ploegh HL. Blood, 121(7). 2013 Feb 14. PMID: 23243279

Cell-specific TLR9 trafficking in primary APCs of transgenic TLR9-GFP mice.
Avalos AM, Kirak O, Oelkers JM, Pils MC, Kim YM, Ottinger M, Jaenisch R, Ploegh HL, Brinkmann MM. J. Immunol., 190(2). 2013 Jan 15. PMID: 23241879



2012 Publications

A catalytically inactive mutant of the deubiquitylase YOD-1 enhances antigen cross-presentation.
Sehrawat S, Koenig PA, Kirak O, Schlieker C, Fankhauser M, Ploegh HL. Blood, 121(7). 2013 Feb 14. PMID: 23243279

Cell-specific TLR9 trafficking in primary APCs of transgenic TLR9-GFP mice.
Avalos AM, Kirak O, Oelkers JM, Pils MC, Kim YM, Ottinger M, Jaenisch R, Ploegh HL, Brinkmann MM. J. Immunol., 190(2). 2013 Jan 15. PMID: 23241879

Autoantibodies produced at the site of tissue damage provide evidence of humoral autoimmunity in inclusion body myositis.
Ray A, Amato AA, Bradshaw EM, Felice KJ, DiCapua DB, Goldstein JM, Lundberg IE, Nowak RJ, Ploegh HL, Spooner E, Wu Q, Willis SN, O'Connor KC. PLoS ONE, 7(10). 2012. PMID: 23071619

IgG1+ ovalbumin-specific B-cell transnuclear mice show class switch recombination in rare allelically included B cells.
Dougan SK, Ogata S, Hu CC, Grotenbreg GM, Guillen E, Jaenisch R, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 109(34). 2012 Aug 21. PMID: 22869725

CD8(+) T cells from mice transnuclear for a TCR that recognizes a single H-2K(b)-restricted MHV68 epitope derived from gB-ORF8 help control infection.
Sehrawat S, Kirak O, Koenig PA, Isaacson MK, Marques S, Bozkurt G, Simas JP, Jaenisch R, Ploegh HL. Cell Rep, 1(5). 2012 May 31. PMID: 22832272

Preparation of unnatural N-to-N and C-to-C protein fusions.
Witte MD, Cragnolini JJ, Dougan SK, Yoder NC, Popp MW, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 109(30). 2012 Jul 24. PMID: 22778432

M13 bacteriophage display framework that allows sortase-mediated modification of surface-accessible phage proteins.
Hess GT, Cragnolini JJ, Popp MW, Allen MA, Dougan SK, Spooner E, Ploegh HL, Belcher AM, Guimaraes CP. Bioconjug. Chem., 23(7). 2012 Jul 18. PMID: 22759232

Attachment of Chlamydia trachomatis L2 to host cells requires sulfation.
Rosmarin DM, Carette JE, Olive AJ, Starnbach MN, Brummelkamp TR, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 109(25). 2012 Jun 19. PMID: 22675117

A viral deubiquitylating enzyme restores dislocation of substrates from the endoplasmic reticulum (ER) in semi-intact cells.
Sanyal S, Claessen JH, Ploegh HL. J. Biol. Chem., 287(28). 2012 Jul 6. PMID: 22619172

The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE(OMe)-FMK).
Davies CW, Chaney J, Korbel G, Ringe D, Petsko GA, Ploegh H, Das C. Bioorg. Med. Chem. Lett., 22(12). 2012 Jun 15. PMID: 22617491

An unconventional role for miRNA: let-7 activates Toll-like receptor 7 and causes neurodegeneration.
Lehmann SM, Krüger C, Park B, Derkow K, Rosenberger K, Baumgart J, Trimbuch T, Eom G, Hinz M, Kaul D, Habbel P, Kälin R, Franzoni E, Rybak A, Nguyen D, Veh R, Ninnemann O, Peters O, Nitsch R, Heppner FL, Golenbock D, Schott E, Ploegh HL, Wulczyn FG, Lehnardt S. Nat. Neurosci., 15(6). 2012 Jun. PMID: 22610069

Development of an influenza virus protein array using Sortagging technology.
Sinisi A, Popp MW, Antos JM, Pansegrau W, Savino S, Nissum M, Rappuoli R, Ploegh HL, Buti L. Bioconjug. Chem., 23(6). 2012 Jun 20. PMID: 22594688

Chemoenzymatic site-specific labeling of influenza glycoproteins as a tool to observe virus budding in real time.
Popp MW, Karssemeijer RA, Ploegh HL. PLoS Pathog., 8(3). 2012. PMID: 22457626

Ubiquitin-like proteins.
van der Veen AG, Ploegh HL. Annu. Rev. Biochem., 81. 2012. PMID: 22404627

Protein ligation in living cells using sortase.
Strijbis K, Spooner E, Ploegh HL. Traffic, 13(6). 2012 Jun. PMID: 22348280

Cholera toxin activates nonconventional adjuvant pathways that induce protective CD8 T-cell responses after epicutaneous vaccination.
Olvera-Gomez I, Hamilton SE, Xiao Z, Guimaraes CP, Ploegh HL, Hogquist KA, Wang L, Jameson SC. Proc. Natl. Acad. Sci. U.S.A., 109(6). 2012 Feb 7. PMID: 22308317

Accessory molecules for Toll-like receptors and their function.
Lee CC, Avalos AM, Ploegh HL. Nat. Rev. Immunol., 12(3). 2012 Mar. PMID: 22301850

Class II MHC self-antigen presentation in human B and T lymphocytes.
Costantino CM, Spooner E, Ploegh HL, Hafler DA. PLoS ONE, 7(1). 2012. PMID: 22299025

Protein quality control in the ER: balancing the ubiquitin checkbook.
Claessen JH, Kundrat L, Ploegh HL. Trends Cell Biol., 22(1). 2012 Jan. PMID: 22055166



2011 Publications

BAT3 guides misfolded glycoproteins out of the endoplasmic reticulum.
Claessen JH, Ploegh HL. PLoS ONE, 6(12). 2011. PMID: 22174835

Identification of host cell factors required for intoxication through use of modified cholera toxin.
Guimaraes CP, Carette JE, Varadarajan M, Antos J, Popp MW, Spooner E, Brummelkamp TR, Ploegh HL. J. Cell Biol., 195(5). 2011 Nov 28. PMID: 22123862

Road to ruin: targeting proteins for degradation in the endoplasmic reticulum.
Smith MH, Ploegh HL, Weissman JS. Science, 334(6059). 2011 Nov 25. PMID: 22116878

Protein quality control in the ER: balancing the ubiquitin checkbook.
Claessen JH, Kundrat L, Ploegh HL. Trends Cell Biol., 22(1). 2012 Jan. PMID: 22055166

Determinants of GBP recruitment to Toxoplasma gondii vacuoles and the parasitic factors that control it.
Virreira Winter S, Niedelman W, Jensen KD, Rosowski EE, Julien L, Spooner E, Caradonna K, Burleigh BA, Saeij JP, Ploegh HL, Frickel EM. PLoS ONE, 6(9). 2011. PMID: 21931713

CD8α(+) dendritic cells are the critical source of interleukin-12 that controls acute infection by Toxoplasma gondii tachyzoites.
Mashayekhi M, Sandau MM, Dunay IR, Frickel EM, Khan A, Goldszmid RS, Sher A, Ploegh HL, Murphy TL, Sibley LD, Murphy KM. Immunity, 35(2). 2011 Aug 26. PMID: 21867928

Dual role of ancient ubiquitous protein 1 (AUP1) in lipid droplet accumulation and endoplasmic reticulum (ER) protein quality control.
Klemm EJ, Spooner E, Ploegh HL. J. Biol. Chem., 286(43). 2011 Oct 28. PMID: 21857022

Fungal recognition is mediated by the association of dectin-1 and galectin-3 in macrophages.
Esteban A, Popp MW, Vyas VK, Strijbis K, Ploegh HL, Fink GR. Proc. Natl. Acad. Sci. U.S.A., 108(34). 2011 Aug 23. PMID: 21825168

Competition by inhibitory oligonucleotides prevents binding of CpG to C-terminal TLR9.
Avalos AM, Ploegh HL. Eur. J. Immunol., 41(10). 2011 Oct. PMID: 21766476

Global gene disruption in human cells to assign genes to phenotypes by deep sequencing.
Carette JE, Guimaraes CP, Wuethrich I, Blomen VA, Varadarajan M, Sun C, Bell G, Yuan B, Muellner MK, Nijman SM, Ploegh HL, Brummelkamp TR. Nat. Biotechnol., 29(6). 2011 Jun. PMID: 21623355

Helicobacter pylori cytotoxin-associated gene A (CagA) subverts the apoptosis-stimulating protein of p53 (ASPP2) tumor suppressor pathway of the host.
Buti L, Spooner E, Van der Veen AG, Rappuoli R, Covacci A, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 108(22). 2011 May 31. PMID: 21562218

Making and breaking peptide bonds: protein engineering using sortase.
Popp MW, Ploegh HL. Angew. Chem. Int. Ed. Engl., 50(22). 2011 May 23. PMID: 21538739

Ubiquitin-dependent control of class II MHC localization is dispensable for antigen presentation and antibody production.
McGehee AM, Strijbis K, Guillen E, Eng T, Kirak O, Ploegh HL. PLoS ONE, 6(4). 2011. PMID: 21533087

End the wasteful tyranny of reviewer experiments.
Ploegh H. Nature, 472(7344). 2011 Apr 28. PMID: 21525890

Sortase A as a tool for high-yield histatin cyclization.
Bolscher JG, Oudhoff MJ, Nazmi K, Antos JM, Guimaraes CP, Spooner E, Haney EF, Garcia Vallejo JJ, Vogel HJ, van't Hof W, Ploegh HL, Veerman EC. FASEB J., 25(8). 2011 Aug. PMID: 21525488

Granulin is a soluble cofactor for toll-like receptor 9 signaling.
Park B, Buti L, Lee S, Matsuwaki T, Spooner E, Brinkmann MM, Nishihara M, Ploegh HL. Immunity, 34(4). 2011 Apr 22. PMID: 21497117

Enzymatic blockade of the ubiquitin-proteasome pathway.
Ernst R, Claessen JH, Mueller B, Sanyal S, Spooner E, van der Veen AG, Kirak O, Schlieker CD, Weihofen WA, Ploegh HL. PLoS Biol., 8(3). 2011 Mar. PMID: 21468303

Sortase-catalyzed transformations that improve the properties of cytokines.
Popp MW, Dougan SK, Chuang TY, Spooner E, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 108(8). 2011 Feb 22. PMID: 21297034

Antigen-specific induction of regulatory T cells in vivo and in vitro.
Daniel C, Ploegh H, von Boehmer H. Methods Mol. Biol., 707. 2011. PMID: 21287335

Derlin-2-deficient mice reveal an essential role for protein dislocation in chondrocytes.
Dougan SK, Hu CC, Paquet ME, Greenblatt MB, Kim J, Lilley BN, Watson N, Ploegh HL. Mol. Cell. Biol., 31(6). 2011 Mar. PMID: 21220515

Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier.
Van der Veen AG, Schorpp K, Schlieker C, Buti L, Damon JR, Spooner E, Ploegh HL, Jentsch S. Proc. Natl. Acad. Sci. U.S.A., 108(5). 2011 Feb 1. PMID: 21209336

The tetraspanin CD82 is specifically recruited to fungal and bacterial phagosomes prior to acidification.
Artavanis-Tsakonas K, Kasperkovitz PV, Papa E, Cardenas ML, Khan NS, Van der Veen AG, Ploegh HL, Vyas JM. Infect. Immun., 79(3). 2011 Mar. PMID: 21149584



2010 Publications

The tetraspanin CD82 is specifically recruited to fungal and bacterial phagosomes prior to acidification.
Artavanis-Tsakonas K, Kasperkovitz PV, Papa E, Cardenas ML, Khan NS, Van der Veen AG, Ploegh HL, Vyas JM. Infect. Immun., 79(3). 2011 Mar. PMID: 21149584

Transnuclear mice with pre-defined T cell receptor specificities against Toxoplasma gondii obtained via SCNT.
Kirak O, Frickel EM, Grotenbreg GM, Suh H, Jaenisch R, Ploegh HL. J Vis Exp. 2010 Sep 30. PMID: 20972401

The HCMV membrane glycoprotein US10 selectively targets HLA-G for degradation.
Park B, Spooner E, Houser BL, Strominger JL, Ploegh HL. J. Exp. Med., 207(9). 2010 Aug 30. PMID: 20713594

Deficiency of antigen-presenting cell invariant chain reduces atherosclerosis in mice.
Sun J, Hartvigsen K, Chou MY, Zhang Y, Sukhova GK, Zhang J, Lopez-Ilasaca M, Diehl CJ, Yakov N, Harats D, George J, Witztum JL, Libby P, Ploegh H, Shi GP. Circulation, 122(8). 2010 Aug 24. PMID: 20697023

Quality and quantity control at the endoplasmic reticulum.
Hegde RS, Ploegh HL. Curr. Opin. Cell Biol., 22(4). 2010 Aug. PMID: 20570125

The transmembrane segment of a tail-anchored protein determines its degradative fate through dislocation from the endoplasmic reticulum.
Claessen JH, Mueller B, Spooner E, Pivorunas VL, Ploegh HL. J. Biol. Chem., 285(27). 2010 Jul 2. PMID: 20435896

Transnuclear mice with predefined T cell receptor specificities against Toxoplasma gondii obtained via SCNT.
Kirak O, Frickel EM, Grotenbreg GM, Suh H, Jaenisch R, Ploegh HL. Science, 328(5975). 2010 Apr 9. PMID: 20378817

Differential regulation of effector- and central-memory responses to Toxoplasma gondii Infection by IL-12 revealed by tracking of Tgd057-specific CD8+ T cells.
Wilson DC, Grotenbreg GM, Liu K, Zhao Y, Frickel EM, Gubbels MJ, Ploegh HL, Yap GS. PLoS Pathog., 6(3). 2010 Mar 19. PMID: 20333242

The Angelman Syndrome protein Ube3A regulates synapse development by ubiquitinating arc.
Greer PL, Hanayama R, Bloodgood BL, Mardinly AR, Lipton DM, Flavell SW, Kim TK, Griffith EC, Waldon Z, Maehr R, Ploegh HL, Chowdhury S, Worley PF, Steen J, Greenberg ME. Cell, 140(5). 2010 Mar 5. PMID: 20211139

Characterization and structural studies of the Plasmodium falciparum ubiquitin and Nedd8 hydrolase UCHL3.
Artavanis-Tsakonas K, Weihofen WA, Antos JM, Coleman BI, Comeaux CA, Duraisingh MT, Gaudet R, Ploegh HL. J. Biol. Chem., 285(9). 2010 Feb 26. PMID: 20042598

A structural element within the HUWE1 HECT domain modulates self-ubiquitination and substrate ubiquitination activities.
Pandya RK, Partridge JR, Love KR, Schwartz TU, Ploegh HL. J. Biol. Chem., 285(8). 2010 Feb 19. PMID: 20007713



2009 Publications

Characterization and structural studies of the Plasmodium falciparum ubiquitin and Nedd8 hydrolase UCHL3.
Artavanis-Tsakonas K, Weihofen WA, Antos JM, Coleman BI, Comeaux CA, Duraisingh MT, Gaudet R, Ploegh HL. J. Biol. Chem., 285(9). 2010 Feb 26. PMID: 20042598

A structural element within the HUWE1 HECT domain modulates self-ubiquitination and substrate ubiquitination activities.
Pandya RK, Partridge JR, Love KR, Schwartz TU, Ploegh HL. J. Biol. Chem., 285(8). 2010 Feb 19. PMID: 20007713

Haploid genetic screens in human cells identify host factors used by pathogens.
Carette JE, Guimaraes CP, Varadarajan M, Park AS, Wuethrich I, Godarova A, Kotecki M, Cochran BH, Spooner E, Ploegh HL, Brummelkamp TR. Science, 326(5957). 2009 Nov 27. PMID: 19965467

The otubain YOD1 is a deubiquitinating enzyme that associates with p97 to facilitate protein dislocation from the ER.
Ernst R, Mueller B, Ploegh HL, Schlieker C. Mol. Cell, 36(1). 2009 Oct 9. PMID: 19818707

Subtilase cytotoxin cleaves newly synthesized BiP and blocks antibody secretion in B lymphocytes.
Hu CC, Dougan SK, Winter SV, Paton AW, Paton JC, Ploegh HL. J. Exp. Med., 206(11). 2009 Oct 26. PMID: 19808260

A broad screen for targets of immune complexes decorating arthritic joints highlights deposition of nucleosomes in rheumatoid arthritis.
Monach PA, Hueber W, Kessler B, Tomooka BH, BenBarak M, Simmons BP, Wright J, Thornhill TS, Monestier M, Ploegh H, Robinson WH, Mathis D, Benoist C. Proc. Natl. Acad. Sci. U.S.A., 106(37). 2009 Sep 15. PMID: 19720992

XBP-1-deficient plasmablasts show normal protein folding but altered glycosylation and lipid synthesis.
McGehee AM, Dougan SK, Klemm EJ, Shui G, Park B, Kim YM, Watson N, Wenk MR, Ploegh HL, Hu CC. J. Immunol., 183(6). 2009 Sep 15. PMID: 19710472

A gammaherpesvirus ubiquitin-specific protease is involved in the establishment of murine gammaherpesvirus 68 infection.
Gredmark-Russ S, Isaacson MK, Kattenhorn L, Cheung EJ, Watson N, Ploegh HL. J. Virol., 83(20). 2009 Oct. PMID: 19706716

Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity.
Antos JM, Chew GL, Guimaraes CP, Yoder NC, Grotenbreg GM, Popp MW, Ploegh HL. J. Am. Chem. Soc., 131(31). 2009 Aug 12. PMID: 19610623

Murine B cell response to TLR7 ligands depends on an IFN-beta feedback loop.
Green NM, Laws A, Kiefer K, Busconi L, Kim YM, Brinkmann MM, Trail EH, Yasuda K, Christensen SR, Shlomchik MJ, Vogel S, Connor JH, Ploegh H, Eilat D, Rifkin IR, van Seventer JM, Marshak-Rothstein A. J. Immunol., 183(3). 2009 Aug 1. PMID: 19587008

Cathepsin S regulates class II MHC processing in human CD4+ HLA-DR+ T cells.
Costantino CM, Ploegh HL, Hafler DA. J. Immunol., 183(2). 2009 Jul 15. PMID: 19553543

Ubiquitination, ubiquitin-like modifiers, and deubiquitination in viral infection.
Isaacson MK, Ploegh HL. Cell Host Microbe, 5(6). 2009 Jun 18. PMID: 19527883

XBP-1 regulates signal transduction, transcription factors and bone marrow colonization in B cells.
Hu CC, Dougan SK, McGehee AM, Love JC, Ploegh HL. EMBO J., 28(11). 2009 Jun 3. PMID: 19407814

Site-specific protein labeling via sortase-mediated transpeptidation.
Popp MW, Antos JM, Ploegh HL. Curr Protoc Protein Sci, Chapter 15. 2009 Apr. PMID: 19365788

A straight path to circular proteins.
Antos JM, Popp MW, Ernst R, Chew GL, Spooner E, Ploegh HL. J. Biol. Chem., 284(23). 2009 Jun 5. PMID: 19359246

Editorial overview.
Ploegh H, Amigorena S. Curr. Opin. Immunol., 21(1). 2009 Feb. PMID: 19342209

Ubiquitin C-terminal electrophiles are activity-based probes for identification and mechanistic study of ubiquitin conjugating machinery.
Love KR, Pandya RK, Spooner E, Ploegh HL. ACS Chem. Biol., 4(4). 2009 Apr 17. PMID: 19256548

Substrate filtering by the active site crossover loop in UCHL3 revealed by sortagging and gain-of-function mutations.
Popp MW, Artavanis-Tsakonas K, Ploegh HL. J. Biol. Chem., 284(6). 2009 Feb 6. PMID: 19047059



2008 Publications

Substrate filtering by the active site crossover loop in UCHL3 revealed by sortagging and gain-of-function mutations.
Popp MW, Artavanis-Tsakonas K, Ploegh HL. J. Biol. Chem., 284(6). 2009 Feb 6. PMID: 19047059

A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway.
Schlieker CD, Van der Veen AG, Damon JR, Spooner E, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 105(47). 2008 Nov 25. PMID: 19017811

Profiling antibody responses by multiparametric analysis of primary B cells.
Story CM, Papa E, Hu CC, Ronan JL, Herlihy K, Ploegh HL, Love JC. Proc. Natl. Acad. Sci. U.S.A., 105(46). 2008 Nov 18. PMID: 19004776

Lipid modification of proteins through sortase-catalyzed transpeptidation.
Antos JM, Miller GM, Grotenbreg GM, Ploegh HL. J. Am. Chem. Soc., 130(48). 2008 Dec 3. PMID: 18989959

Proteolytic cleavage in an endolysosomal compartment is required for activation of Toll-like receptor 9.
Park B, Brinkmann MM, Spooner E, Lee CC, Kim YM, Ploegh HL. Nat. Immunol., 9(12). 2008 Dec. PMID: 18931679

The CD8 T-cell response against murine gammaherpesvirus 68 is directed toward a broad repertoire of epitopes from both early and late antigens.
Gredmark-Russ S, Cheung EJ, Isaacson MK, Ploegh HL, Grotenbreg GM. J. Virol., 82(24). 2008 Dec. PMID: 18922872

Parasite stage-specific recognition of endogenous Toxoplasma gondii-derived CD8+ T cell epitopes.
Frickel EM, Sahoo N, Hopp J, Gubbels MJ, Craver MP, Knoll LJ, Ploegh HL, Grotenbreg GM. J. Infect. Dis., 198(11). 2008 Dec 1. PMID: 18922097

Differential effects of Usp14 and Uch-L1 on the ubiquitin proteasome system and synaptic activity.
Walters BJ, Campbell SL, Chen PC, Taylor AP, Schroeder DG, Dobrunz LE, Artavanis-Tsakonas K, Ploegh HL, Wilson JA, Cox GA, Wilson SM. Mol. Cell. Neurosci., 39(4). 2008 Dec. PMID: 18771733

SEL1L nucleates a protein complex required for dislocation of misfolded glycoproteins.
Mueller B, Klemm EJ, Spooner E, Claessen JH, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 105(34). 2008 Aug 26. PMID: 18711132

Live imaging of cysteine-cathepsin activity reveals dynamics of focal inflammation, angiogenesis, and polyp growth.
Gounaris E, Tung CH, Restaino C, Maehr R, Kohler R, Joyce JA, Ploegh HL, Plough HL, Barrett TA, Weissleder R, Khazaie K. PLoS ONE, 3(8). 2008 Aug 13. PMID: 18698347

Concurrent detection of secreted products from human lymphocytes by microengraving: cytokines and antigen-reactive antibodies.
Bradshaw EM, Kent SC, Tripuraneni V, Orban T, Ploegh HL, Hafler DA, Love JC. Clin. Immunol., 129(1). 2008 Oct. PMID: 18675591

The known unknowns of antigen processing and presentation.
Vyas JM, Van der Veen AG, Ploegh HL. Nat. Rev. Immunol., 8(8). 2008 Aug. PMID: 18641646

UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes.
Kim YM, Brinkmann MM, Paquet ME, Ploegh HL. Nature, 452(7184). 2008 Mar 13. PMID: 18305481

Lysosomal cysteine and aspartic proteases are heterogeneously expressed and act redundantly to initiate human invariant chain degradation.
Costantino CM, Hang HC, Kent SC, Hafler DA, Ploegh HL. J. Immunol., 180(5). 2008 Mar 1. PMID: 18292509

Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers.
Grotenbreg GM, Roan NR, Guillen E, Meijers R, Wang JH, Bell GW, Starnbach MN, Ploegh HL. Proc. Natl. Acad. Sci. U.S.A., 105(10). 2008 Mar 11. PMID: 18245382

N-linked glycosylation does not impair proteasomal degradation but affects class I major histocompatibility complex presentation.
Kario E, Tirosh B, Ploegh HL, Navon A. J. Biol. Chem., 283(1). 2008 Jan 4. PMID: 17951257



2007 Publications

A herpesvirus ubiquitin-specific protease is critical for efficient T cell lymphoma formation.
Jarosinski K, Kattenhorn L, Kaufer B, Ploegh H, Osterrieder N. Proc. Natl. Acad. Sci. U.S.A., 104(50). 2007 Dec 11. PMID: 18056809

Bridging B cell and T cell recognition of antigen.
Ploegh HL. J. Immunol., 179(11). 2007 Dec 1. PMID: 18025158

Hidde Ploegh: immunologist, journeyman. Interview by Nicole LeBrasseur.
Ploegh H. J. Cell Biol., 179(3). 2007 Nov 5. PMID: 17984316

N-linked glycosylation does not impair proteasomal degradation but affects class I major histocompatibility complex presentation.
Kario E, Tirosh B, Ploegh HL, Navon A. J. Biol. Chem., 283(1). 2008 Jan 4. PMID: 17951257

Mechanisms, biology and inhibitors of deubiquitinating enzymes.
Love KR, Catic A, Schlieker C, Ploegh HL. Nat. Chem. Biol., 3(11). 2007 Nov. PMID: 17948018

Sortagging: a versatile method for protein labeling.
Popp MW, Antos JM, Grotenbreg GM, Spooner E, Ploegh HL. Nat. Chem. Biol., 3(11). 2007 Nov. PMID: 17891153

The peptide cargo of class I molecules: not just passive passengers.
Ploegh HL. J. Immunol., 179(7). 2007 Oct 1. PMID: 17878322

Differential priming of CD8 and CD4 T-cells in animal models of autoimmune hepatitis and cholangitis.
Derkow K, Loddenkemper C, Mintern J, Kruse N, Klugewitz K, Berg T, Wiedenmann B, Ploegh HL, Schott E. Hepatology, 46(4). 2007 Oct. PMID: 17657820

Screen for ISG15-crossreactive deubiquitinases.
Catic A, Fiebiger E, Korbel GA, Blom D, Galardy PJ, Ploegh HL. PLoS ONE, 2(7). 2007 Jul 25. PMID: 17653289

A lipid-based model for the creation of an escape hatch from the endoplasmic reticulum.
Ploegh HL. Nature, 448(7152). 2007 Jul 26. PMID: 17653186

A functional ubiquitin-specific protease embedded in the large tegument protein (ORF64) of murine gammaherpesvirus 68 is active during the course of infection.
Gredmark S, Schlieker C, Quesada V, Spooner E, Ploegh HL. J. Virol., 81(19). 2007 Oct. PMID: 17634221

Sequence and structure evolved separately in a ribosomal ubiquitin variant.
Catic A, Sun ZY, Ratner DM, Misaghi S, Spooner E, Samuelson J, Wagner G, Ploegh HL. EMBO J., 26(14). 2007 Jul 25. PMID: 17599068

TLRs bent into shape.
Kim YM, Brinkmann MM, Ploegh HL. Nat. Immunol., 8(7). 2007 Jul. PMID: 17579642

Empty class II major histocompatibility complex created by peptide photolysis establishes the role of DM in peptide association.
Grotenbreg GM, Nicholson MJ, Fowler KD, Wilbuer K, Octavio L, Yang M, Chakraborty AK, Ploegh HL, Wucherpfennig KW. J. Biol. Chem., 282(29). 2007 Jul 20. PMID: 17525157

Tubulation of class II MHC compartments is microtubule dependent and involves multiple endolysosomal membrane proteins in primary dendritic cells.
Vyas JM, Kim YM, Artavanis-Tsakonas K, Love JC, Van der Veen AG, Ploegh HL. J. Immunol., 178(11). 2007 Jun 1. PMID: 17513769

The mouse polyubiquitin gene UbC is essential for fetal liver development, cell-cycle progression and stress tolerance.
Ryu KY, Maehr R, Gilchrist CA, Long MA, Bouley DM, Mueller B, Ploegh HL, Kopito RR. EMBO J., 26(11). 2007 Jun 6. PMID: 17491588

Chemical biology: dressed-up proteins.
Grotenbreg G, Ploegh H. Nature, 446(7139). 2007 Apr 26. PMID: 17460654

The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling.
Brinkmann MM, Spooner E, Hoebe K, Beutler B, Ploegh HL, Kim YM. J. Cell Biol., 177(2). 2007 Apr 23. PMID: 17452530

ElaD, a Deubiquitinating protease expressed by E. coli.
Catic A, Misaghi S, Korbel GA, Ploegh HL. PLoS ONE, 2(4). 2007 Apr 18. PMID: 17440617

Apicomplexan UCHL3 retains dual specificity for ubiquitin and Nedd8 throughout evolution.
Frickel EM, Quesada V, Muething L, Gubbels MJ, Spooner E, Ploegh H, Artavanis-Tsakonas K. Cell. Microbiol., 9(6). 2007 Jun. PMID: 17371404

Loss of Aire-dependent thymic expression of a peripheral tissue antigen renders it a target of autoimmunity.
Gavanescu I, Kessler B, Ploegh H, Benoist C, Mathis D. Proc. Natl. Acad. Sci. U.S.A., 104(11). 2007 Mar 13. PMID: 17360567

Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes.
Schlieker C, Weihofen WA, Frijns E, Kattenhorn LM, Gaudet R, Ploegh HL. Mol. Cell, 25(5). 2007 Mar 9. PMID: 17349955

Chemical probes for the rapid detection of Fatty-acylated proteins in Mammalian cells.
Hang HC, Geutjes EJ, Grotenbreg G, Pollington AM, Bijlmakers MJ, Ploegh HL. J. Am. Chem. Soc., 129(10). 2007 Mar 14. PMID: 17305342

AID-/-mus-/- mice are agammaglobulinemic and fail to maintain B220-CD138+ plasma cells.
Kumazaki K, Tirosh B, Maehr R, Boes M, Honjo T, Ploegh HL. J. Immunol., 178(4). 2007 Feb 15. PMID: 17277124